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KMID : 0603519980030020107
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Journal of Korean Association of Cancer Prevention
1998 Volume.3 No. 2 p.107 ~ p.114
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Bile-Acid Induced Apoptosis in HL-60 Leukemia Cells
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Kim Kwang-Rok
Kang Chang-Mo
Baek Jin-Hyen
Lee You-Mie
Kim Kyu-Won
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Abstract
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We investigated that lithocholic acid (LCA) or ursodeoxycholilc acid (UDCA), components of bile salt, induced apoptosis in HL-60 cells. The growth of HL-60 cells was reduced by these compounds in a time- and a concentration-dependent manners. Moreover, the degree of DNA fragmentation was increased depending on the treatment time and molar concentration of LCA or UDCA on agarose gel electrophoresis. Morpllologically, we examined the formation of membrane-enclosed apoptotic bodies in LCA or UDCA-treated HL-60 cells by haematoxylin/eosin staining. The expression of c-myc was down-regulated after 24 h treatment of LCA or UDCA In case of bcl-2, there was no change of the expression after 24 h treatment of LCA or UDCA. The DNA fragmentation was reduced when HL-60 cells were treated with 0.5 μM cycloheximide (CHX) and LCA or UDCA. Therefore, bile acids, LCA and UDCA, induced apoptosis in HL-60 cells, which required dr novo protein synthesis.
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KEYWORD
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HL-60 leukemia cells, Apoptosis, LCA, UDCA, c-myc
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